2002

 

A survey on water pollution by Endocrine Disrupter Compounds:

Monitoring of Organochlorine Pesticides, Phenols and Phthalates  In The Coastal Hydrosphere of Indonesia

 

Dewi Ratnaningsih, M Helmy, Bagus BE, Nety W, Heni P.

Environmental Management Center

Kawasan PUSPIPTEK Serpong Tangerang

Indonesia

 

 

ABSTRACT

 

Monitoring of Endocrine Disrupter compounds (EDCs) in the coastal hydrosphere of  Indonesia was carried out to investigate environmental water contamination by persistent organochlorine insecticides, phenols and phthalate compounds.  Surface river and sea water  from four cities  in Jawa Island including Jakarta, Bogor, Semarang and Surabaya were selected as sampling locations.  13 river water  samples and 6 sea water samples were collected in August 2001 represented  dry season and  similar sampling location with the same sample amount  were collected in November 2001 as represented rainy season.  The target compounds for analysis  consist of a group of   organochlorine insecticides including  a-BHC,  g-BHC, d-BHC  Heptachlor, Aldrin, Dieldrin, Endrin, op-DDE, pp-DDE, op-DDD, pp-DDD , op-DDT, pp-DDT; a group of  phenol compounds including 4-t-Butylphenol, 2-4-Dichlorophenol, 4-n-Butylphenol, 4-n-Pentylphenol, 4-n-Hexylphenol, 4-t-Octylphenol, 4-n-Heptylphenol, 4-n-Nonylphenol, 4-n-Octylphenol, Pentachlorophenol, Bhisphenol-A and a group of phthalate compounds including  Diethyl phthalates, Di n-butyl phthalate, Di 2-ethyl hexyl adipate, Di 2-ethyl hexyl phthalate.

 

The monitoring result indicated that some organochlorine compounds, phenols and phthalates were detected in river and sea water samples in trace level amount.  For Heptachlor, d-BHC, Aldrin and DDE  were mostly undetected in many samples  neither in river nor sea water.  DDT and its derivates were  still detected with the value  varied from nd to 122 ppt.  The other organochlorine compounds were detected with the highest value was 23.37 ppt.  Phenol compounds were also detected in almost all of sampling locations.  The highest value for each location was mostly detected in the form of nonylphenol. The range of nonylphenol  concentration were detected from  nd to 8659 ppt.  Bhisphenol-A was detected with the range  from 0.06 to 230 ppt.  Other phenol compounds had a range from nd to 44.24 ppt.  Phthalates were also detected in almost all of sampling sites with the value ranged between nd and 12.92 ppb  Diethyl phthalate and Di-2-etyl hexyl adipate were found to be  higher  mostly in dry season.  Although the result indicated that the concentration of EDCs in the river and sea water  were relatively low, it is necessary to continue this monitoring activities in order to obtain the trend of EDCs pollutant level in the coastal hydrosphere of Indonesia  .

 

 

INTRODUCTION

The uses of toxic organic compounds for industrial and agricultural activities result in the discharge of such contaminants to the environment. The potential adverse impact to living organisms, particularly human being is of particular concern due to the existence of these organic pollutants.

 

The pollution by Endocrine Disrupter Compounds such as  organochlorine, phenol and phthalate compounds in the environment should be paid more attention. Since rivers are   still used as the final discharge of  the wastes from domestic  and industrial activities , therefore the existence of such compound should be awared due to  the negative impact to the environment and human health.    The multipurpose function of the river for human needs also lead to  some necessary  action to be made  in order to prevent  more dangerous chemical from entering the river body, considering  some major  rivers are still used as the source of water supply system.

 

In Indonesia, organochlorine  compounds    had been banned since more than 10 years ago.  However, the residue of organochlorine  still exist  in the environment  due to the persistent  characteristic of this compound.  The previous survey indicated that several organochlorine compounds had  contaminated the river  in ppt level and sediment or soil in ppb level.   Phenol and phthalate compounds also contribute  as the potential pollutant to the water, because  those  source of compounds  are still used to fulfill human needs either for domestic or industrial activities.   

 

The objective of this monitoring  is to identify the current level of several organic pollutants in the river and sea water.  The target compounds for analysis  consist of a group of   organochlorine insecticides including  a-BHC,  g-BHC, d-BHC  Heptachlor, Aldrin, Dieldrin, Endrin, op-DDE, pp-DDE, op-DDD, pp-DDD , op-DDT, pp-DDT; a group of  phenol compounds including 4-t-Butylphenol, 2-4-Dichlorophenol, 4-n-Butylphenol, 4-n-Pentylphenol,  4-n-Hexylphenol,  4-t-Octylphenol,   4-n-Heptylphenol,    4-n-Nonylphenol,    4-n-Octylphenol,  Pentachlorophenol,  Bhisphenol-A  and  a group of phthalate compounds

including  Diethyl phthalates, Di n-butyl phthalate, Di 2-ethyl hexyl adipate, Di 2-ethyl hexyl phthalate.  Continuous monitoring is necessary to be carried out  in order to  get representative data for assessing  organic pollutant condition in the environment.


METHODOLOGY

 

The monitoring activities on organochlorines, phthalates and phenols contamination in environment were carried out from August 2001 to November 2001.    Thirty eight- samples were collected from five cities  which consisted  of river and sea water samples.  Four main rivers  in Java Island and a secondary river located in industrial area were selected for sampling point. Other samples were collected from sea water which was taken in Jakarta Bay, Madura Strait Surabaya and Jawa Sea Semarang.  The main river selected for sampling point included  Ciliwung River at Bogor-Jakarta city, Banjir Kanal Barat and Banjir kanal Timur River at Semarang and  Surabaya River as well as  Rungkut River at Surabaya.  Sampling  data sheet information was shown  in the appendix.  After collecting the samples, then were placed  in the dark glass bottle and kept at cooling room at 4 oC.

 

Apparatus

1.      GCMS QP-5000 (Shimadzu) equipped with chromatographic column-capillary  DB-1, 30m x 0.32 mm id x 0.25 mm of film thickness

2.      Rotary evaporator

3.      Shaker

4.      Separatory funnel 1000, 2000 ml

5.      Erlenmeyer flask

 

Reagents

1.      Hexane for residue analyses grade

2.      Acetone for residue analyses grade

3.      Dichloromethane

4.      Silica-gel cartridge 500 mg (varian)

5.      Sodium Sulfate Anhydrous

6.      Sodium Chloride

7.      Organochlorine insecticides standard including a-HCH, g-HCH, d-HCH, Aldrin, Heptachlor, Endrin, Dieldrin, Metoxichlor, o,p’-DDD, p,p’-DDD, o,p’-DDE, p,p’-DDE, o,p’-DDT, p,p’-DDT.


8.      Phenol standards including 4-t-Butylphenol, 2,4-Dichlorophenol, 4-n-Butylphenol, 4-n-Pentylphenol, 4-n-Hexylphenol, 4-n-Heptylphenol, 4-t-Octylphenol, 4-n-nonyl-phenol, 4-n-Octylphenol, Pentachlorophenol, Bhisphenol-A, Bhisphenol-A d14.

9.      Phthalates Standards including Diethyl phthalates, Di n-butyl phthalate, Di 2-ethyl hexyl adipate, Di 2-ethyl hexyl phthalate.

 

Sample Extraction  for Organochlorine compounds.

1L water sample was placed into 2 L separating funnel and added by  30 g of sodium chloride.  For extraction purpose, water sample was added by 50 ml of n- hexane and shaken for 10 minutes by shaker.  The hexane layer was transferred to erlenmeyer flask.  The water layer was re-extracted with 50 ml-n-hexane.  The extract result was added by sodium sulfate anhydrous in order to eliminate water content from organic layer.  The extraction layer was concentrated and cleaned-up by using sep-pack cartridge  J-18.  The solution was  added by IS and adjusted up to 1 ml and then analyzed by using GCMS.

 

Sample Extraction  for Phenol compounds.

500 ml of water sample was placed into 1 L of separating funnel and adjusted pH at value 2-3.  For extraction purpose, 50 ml Dichloromethane and 30 g of sodium chloride were added and then shaken for 10 minutes.   Extraction was performed two times.  Surrogate compound was included for quality control purposes.  The organic layer resulted from extraction process was transferred to erlenmeyer flask and removed the water content by adding sodium sulfate anhydrous.  The organic layer was concentrated until the volume less than 0.5 ml and added by 100 ml BSTFA for derivative reaction.  Solution was allowed to stand for 1 hour and added by IS and filled up into 1 ml by dichloromethane.  Than 2 ml solution was injected into GCMS.

 

Sample Extraction  for Phthalate compounds.

100 ml water sample was placed into 100 ml volumetric flask and added by 5 ml hexane.  Surrogate and Internal Standard were included for quality control.  For extraction purpose, water sample was shaken for 1 minute and then transferred  to 1 ml of hexane  layer from volumetric flask into vial for auto sampler.  2 ml of solution was injected  into GCMS for determination of phthalate concentration.

RESULT AND DISCUSSION

 

Quality control  for analysis was assessed  by conducting recovery test for each parameter. The recovery test was performed by spiking each standard solution for phthalates, phenols and organochlorines in distillate water and then analyzed for each compound.  The recovery test result for group of phthalates showed  that value  ranged  between  94 and102 % ; For group of phenol between 90 and 106 %;  and  for  group of organochlorine between  77 and  101%.

 

River and sea water samples were analyzed to identify the existence of  EDCs including phthalate, organochlorine, and phenol compounds in environment.  The concentration of phthalate compounds  in river and sea water in Indonesia  are shown in table 1.

 

Table 1. Concentration of Phthalate Compounds in River and Sea Water in Indonesia

 

SAMPLING  SITES

COMPONENT AND CONCENTRATION ( ppb )

City

Code

 

Diethyl phthalate  ( DEP)   

Di n-butyl phthalate (DBP)

Di 2-ethyl hexyl adipate (DOA)

Di 2-ethyl hexyl phthalate (DEHP)

Dry season

Wet season

Dry season

Wet season

Dry season

Wet season

Dry season

Wet season

B

O

G

O

R-J

   A

   K

   A

   R

   T

   A

WC1

Ciawi-Bogor

0.70

0.19

0.72

0.38

0.52

0.30

0.64

0.78

WC2

Tajur-Bogor

0.64

0.17

1.24

0.13

0.81

0.38

1.64

2.28

WC3

Botanical Garden-Bogor

3.54

0.71

1.94

0.40

0.34

0.34

1.37

1.47

WC4

Kalibata-Jakarta

1.00

1.36

1.30

3.34

0.15

0.81

2.46

2.09

WC5

Gn. Sahari -Jakarta

1.49

12.92

1.24

4.40

0.17

0.25

1.42

5.52

SC6

Marina Beach-Jakarta

0.43

0.15

0.36

2.24

0.24

0.24

0.43

3.19

SP1

Tanjung Priuk Harbour.

0.95

0.20

0.80

1.05

0.59

0.23

1.89

4.11

S

U

R

A

B

A

Y

A

WR1

Rungkut

0.51

1.65

3.67

1.03

0.34

0.32

6.89

1.31

WB1

Gunungsari

1.37

0.13

11.77

0.91

0.43

nd

6.35

1.04

WB2

Wonokromo

2.53

0.19

10.88

0.92

0.52

nd

4.55

0.74

WB3

Jembatan Merah

1.82

0.62

6.10

1.08

2.25

0.29

5.42

3.14

SB4

T. Perak Harbour

0.59

0.03

1.88

2.21

0.08

0.36

2.57

4.57

SJ1

Kenjeran Beach

0.53

0.03

3.08

1.27

0.17

nd

3.82

1.72

S

E

M

A

R

A

N

G

WT1

BKT Mojopahit up

4.83

3.97

2.07

2.27

nd

0.47

1.86

2.86

WT2

BKT down

6.72

1.23

2.61

2.07

0.04

0.32

2.79

2.11

WK1

PDAM(BKB) up

0.51

0.92

1.59

3.56

nd

0.59

2.44

3.24

WK2

Katamso (BKB)

0.41

0.41

1.26

3.39

0.12

0.25

1.16

2.23

SK3

Marina Beach

0.66

0.28

1.73

1.45

0.12

nd

4.70

1.24

SM1

Tanjung Emas 

0.73

0.16

2.91

1.53

0.36

nd

2.52

0.86

Note:

             I                  : dry season                          W            : river water                    nd   : not detected

             II                 : Rainy season                      S              : sea water

            

 

Phthalate compounds were detected in almost all of river and sea water samples. The concentrations were varied  from nd to  12.92 ppb.  Dietyl phthalate  (DEP) were detected in dry season with the concentration ranged from 0.41 to 6.72 ppb. In wet season, those compounds were found with the range from  0.03 to 12.92 ppb.  The highest value  of DEP  (12.92 ppb) was detected at sampling site WC5 (Ciliwung river) located in downtown of Jakarta city.  This sampling site was located at downstream of Ciliwung river about 5 km  up stream from the river mouth of Jakarta bay.    Mostly, the highest concentration of several phthalates  including  DBP and DEHP were detected in those sampling sites,  particularly in rainy season.   This fact  can probably be explained that during  rainy season, contaminated sediment or soil entered  to the river water which was resulted from heavy  rainfall.  Therefore,   some of the phthalates which were  trapped in the soil and sediment will be then flown to the surface river water.   Additionally, dense activities of human being at downtown area had generated wastes which would flow into the river and eventually be accumulated in downstream.

 

Table 2. Comparison of the Concentration Average of Phthalate Compounds in Dry     

             and Rainy Season

 

PHTHALATE COMPOUNDS  (ppb)

DEP

DBP

DOA

DEHP

Range

mean

Range

mean

Range

mean

Range

mean

DRY

0.41-6.72

1.58

0.36-11.77

3.01

nd-2.25

0.38

0.43-6.89

2.89

RAINY

0.03-12.92

1.28

0.13-4.40

1.77

nd-0.81

0.27

0.74-5.52

2.34

 

DBP was found  with the minimum concentration 0.36 ppb  and maximum value 11.77 ppb in dry season, while the concentration of DBP varied from  0.13 to 4.40 ppb in wet season..  DOA was detected  lower than other phthalate compounds with the average value 0.38 ppb in dry season and 0.27 ppb in rainy season. Meanwhile DEHP had concentration ranged between 0.43 and 6.89 ppb in dry season and 0.74 to 5.52 ppb in rainy season.   Eventhough, the highest concentration of phthalates was detected in rainy season, from the average value  (table 2) it can be assumed  that phthalate  concentration were relatively   higher in dry season than in rainy season. It is correspondent to  the assumption that in the rainy season, the river water will be diluted due to  the rainfall,  consequently the  concentration will be lower in rainy season.   For the  compound which was detected in traceable amount there was no significantly different result between dry and rainy season.  In general, the result  of phthalate compounds  in surface water sample were detected in relatively low concentration, partly because of both physical-chemical and biological degradation factors.

 

Table 3.  Concentration  of Organochlorine Compounds in the River and Sea Water in 

                Indonesia 

 

SAMPLING  SITES

COMPONENT AND CONCENTRATION ( ppt )

City

CODE

a-HCH

g-HCH

d-HCH

Hep-tachlor

Al- drin

Die-ldrin

En-drin

o-p’ DDE

p-p’ DDE

o,p-DDD

p,p-DDD

o,p-DDT

p,p-DDT

Metoxi-chlor

 

 

S

U

R

A

B

A

Y

A

WR1-I

3.29

4.75

nd

5.29

nd

nd

3.31

nd

nd

0.32

2.90

3.01

3.49

4.61

WR1-II

4.68

3.79

nd

nd

nd

nd

0.51

nd

nd

0.51

0.86

3.88

1.61

nd

WB1-I

nd

4.18

nd

nd

nd

1.06

4.83

nd

97.63

29.97

21.81

114.9

121.6

13.93

WB1-II

nd

2.24

nd

nd

nd

nd

nd

nd

nd

0.72

0.75

2.97

1.32

nd

WB2-I

nd

2.86

nd

nd

nd

0.58

nd

nd

nd

0.50

2.87

nd

3.41

4.53

WB2-II

nd

2.65

nd

nd

nd

nd

nd

nd

nd

0.51

0.94

9.75

1.70

2.73

WB3-I

2.22

5.58

nd

nd

nd

nd

3.03

nd

0.04

0.61

2.84

3.42

3.20

4.81

WB3-II

2.14

2.87

nd

nd

nd

nd

nd

nd

nd

0.55

0.79

4.24

1.48

nd

SB4-I

nd

3.22

nd

nd

1.09

nd

nd

nd

nd

0.54

3.17

2.48

3.40

nd

SB4-II

nd

1.33

nd

nd

nd

nd

nd

nd

nd

nd

0.67

0.74

1.32

2.68

SJ1-I

1.30

3.94

nd

nd

nd

nd

nd

nd

0.48

nd

2.80

2.34

3.48

4.45

SJ1-II

nd

2.15

nd

nd

nd

nd

nd

nd

nd

0.51

0.65

0.83

1.62

nd

 

 

S

E

M

A

R

A

N

G

WT1-I

12.08

4.69

nd

nd

nd

0.17

4.85

nd

0.08

0.61

3.89

2.91

nd

nd

WT1-II

7.73

2.32

3.81

nd

nd

nd

0.77

nd

nd

0.57

1.02

0.92

nd

nd

WT2-I

7.03

15.69

nd

nd

1.54

nd

nd

nd

nd

0.90

3.47

3.60

nd

nd

WT2-II

4.88

18.21

nd

nd

nd

nd

4.92

nd

nd

0.61

0.75

0.85

nd

nd

WK1-I

1.51

4.66

nd

nd

nd

nd

nd

nd

nd

0.36

2.75

nd

3.14

4.48

WK1-II

0.38

2.58

nd

nd

nd

nd

nd

nd

nd

0.70

2.38

0.80

1.87

nd

WK2-I

1.63

4.21

nd

nd

2.05

nd

nd

nd

nd

0.38

2.81

nd

nd

nd

WK2-II

0.74

2.50

nd

nd

nd

nd

nd

nd

nd

0.48

0.73

0.55

1.81

2.87

SK3-I

1.21

6.16

nd

nd

nd

nd

3.43

nd

nd

0.39

2.82

nd

3.17

nd

SK3-II

0.46

3.63

nd

nd

nd

nd

0.01

nd

nd

0.51

0.70

0.60

nd

2.76

SM1-I

nd

nd

nd

nd

nd

nd

nd

nd

nd

nd

nd

nd

nd

nd

SM1-II

0.64

2.12

nd

nd

nd

nd

5.04

nd

nd

0.53

0.72

0.55

1.48

nd

B

O

G

O

R

 

&

 

J

A

K

A

R

T

A

WC1-I

1.49

5.43

nd

nd

1.51

nd

nd

nd

nd

0.92

2.94

2.73

3.37

4.50

WC1-II

nd

1.34

nd

nd

nd

nd

nd

nd

0.10

1.22

1.19

0.79

1.68

2.90

WC2-I

1.79

7.19

nd

nd

nd

nd

5.08

nd

0.57

1.86

2.99

3.68

nd

nd

WC2-II

0.04

1.89

nd

nd

nd

nd

nd

nd

nd

0.47

0.70

0.57

1.29

nd

WC3-I

4.34

7.34

nd

nd

nd

nd

nd

nd

nd

0.69

3.26

2.57

3.76

4.71

WC3-II

0.53

1.36

nd

nd

nd

nd

nd

nd

nd

0.53

0.72

0.60

1.39

nd

WC4-I

3.02

13.67

nd

nd

nd

0.29

3.56

nd

nd

0.97

3.04

4.05

6.24

nd

WC4-II

2.96

0.68

3.25

nd

nd

nd

nd

nd

nd

0.51

0.68

0.66

nd

nd

WC5-I

19.99

6.64

nd

nd

nd

0.32

2.79

nd

0.18

1.77

3.11

4.12

3.30

nd

WC5-II

23.37

nd

3.33

nd

nd

nd

nd

nd

0.68

1.01

0.85

2.22

nd

nd

SC6-I

1.47

7.37

nd

nd

nd

nd

nd

nd

nd

0.85

3.28

2.53

3.49

4.76

SC6-II

0.09

1.82

nd

nd

nd

nd

nd

nd

nd

0.46

0.71

0.73

1.38

nd

SP1-I

1.15

4.71

nd

nd

1.19

nd

2.57

nd

nd

0.74

3.14

2.68

3.43

4.59

SP1-II

0.12

2.96

nd

nd

nd

nd

nd

nd

nd

0.49

0.65

0.78

1.30

nd

Note:

             I  : dry season                          W: river water                                       nd: not detected

             II : Rainy season                      S: sea water

            

The concentration of organochlorine residues in river and sea water sample are shown in table 3.   Several organochlorines  were detected  in almost all sampling sites except for d-BHC and Heptachlor.    The major compounds that were  detected in all sampling sites were µ-BHC, g-BHC, o,p-DDD, p,p-DDD, o,p-DDT, p,p-DDT and metoxichlor.

 

µ-BHC  was detected in dry season  with the concentration varied from nd to 19.99 ppt  and the average 3.34 ppt.  In wet season, this compound  was found  in the ranges from nd to   23. 37  ppt with the average  was 2.56 ppt.   The average concentration of  µ-BHC has considered to be higher in dry season than wet season.  The highest concentration of µ-BHC  was detected in wet season with the value of 23.37 ppt followed by  dry season with the value 19.99 ppt.  Both concentrations  were detected in sampling site  no WC 5 which was situated at downstream of Ciliwung River Jakarta.

 

Lindane or g-BHC were also detected in all sampling sites except for sampling site  no SM1 in dry season  and WC5 in wet season.  The highest concentration was detected in sampling site WT2, either in wet  or dry season with the value respectively  18.21 and  15.69 ppt.  This sampling site was  downstream of Banjir Kanal  Timur River located in Semarang city.  The river in this sampling site was very dirty because this  sampling site  was  near by   traditional market and residential areas.

 

Mostly DDT and its derivate were detected in this survey,  except for DDE.  O,p- DDE was not found in  all sampling sites. However  p,p-DDE was  still detected in six sampling sites with the range from 0.08 to 97.63 ppt.  The highest concentration for p,p-DDE was detected in dry season at sampling site no. WB1 (assumed as an  upstream of Surabaya River in Surabaya city).    In dry season, the highest concentration for o,p-DDD, p,p-DDD, o,p-DDT and p,p-DDT were also detected in sampling site WBI with the values respectively 29.97 ppt, 21.81 ppt, 114.9 ppt and 121.6 ppt.  This sampling site was actually  not considered as a real upstream of this river. However, this site was assumed to be upstream because the location of this sampling site was located in the border of Surabaya city.  Before entering  Surabaya city, the river of this sampling site already received some burdens  from other city closed to Surabaya . This sampling site can be considered   as a downstream from previous city which passed through  this river.  Besides, there were some paddy fields  located near the river before entering this sampling site.  Therefore,  the concentration of some organochlorine here was  relatively high compared to other sites.  Eventhough,  during  dry season this site give contribution as the highest  concentration of organochlorine, but in wet season the concentration of those organochlorine drastically reduced down to the range nd to 1.32 ppt.

 

P,p-DDT was found in the river and sea water samples with the concentration ranged from nd to 121.6 ppt with the average 8.87 in dry season.  In rainy season the range of this  compound was from nd to 1.81 ppt  with the average concentration 1.12 ppt.  The average concentration of o,p- DDT in dry and wet season were similar to  p,p-DDT with the value  respectively 8.17 ppt and  1.74 ppt.   O,p-DDD  and p,p-DDD were also found in dry  and wet season with  the concentration ranges  respectively from nd and 29.97 ppt  (dry season) ;  nd to 1.22 ppt (wet season)   and  from nd to21.81 ppt  (dry season) ;  0.65 to 2.38  ppt (wet season). 

 

With respect to average concentration of each organochlorine,  it can be stipulated that in dry season the concentration of organochlorine  was higher than wet season.  Sampling site no WB1 in Surabaya city  was more polluted than other sites followed by sampling site no WC5 (downstream Ciliwung River)  Jakarta  and  sampling site no. WT2 (downstream Banjir Kanal Timur River) Semarang.

 

In general,  the river and coastal water in Surabaya, Semarang, Bogor and Jakarta  were  polluted by several organochlorine compounds, eventhough the concentration was detected in traceable amount.   The existence of DDT and other persistent compounds in the water samples provided evidence of the continuing influence of those compounds which had previously been used in the past.

 

Phenol  concentrations in river and sea water  are indicated in table 3.  Mostly, all of sampling sites  were contaminated by  phenol compounds eventhough it was detected in trace amount level.  Regarding the average of each phenol compound, the highest concentration of phenol compound was found in the form of  4-n-Nonylphenol followed by  Bhisphenol-A, 2-4-Dichlorophenol and  4-t-Butylphenol.   

 

4-n-Nonylphenol was detected in all of sampling sites except for sampling site no. SM1 (sea  water from the Marina Beach Semarang)  with the concentration range varied from 532 to 8720 ppt  in dry season and  in wet season varied from 304 to 8659 ppt.   The highest concentration of this compound was  detected   in sampling site no. SC6 ( sea water about 1 km  away  from Ciliwung river mouth) followed by sampling site no. WC5  ( downstream  Ciliwung  River  about 5  km downward  from Ciliwung  river mouth ).       4-n-nonylphenol  was generally used as an ingredient of surfactant in various household and industrial uses, therefore the concentration of this compound in the water body was relatively high  when compared to other compounds. 

 

Table 4.  Concentration  of Phenol Compounds in the River and Sea Water in 

               Indonesia 

 

SAMPLING  SITES

COMPONENT AND CONCENTRATION (ppt)

City

Code

 

4-t-BP

2-4-DP